yugO [2018-12-27 18:05:07]

potassium channel protein (exporter)

Locus: BSU31322

Isoelectric point: 7.00

Molecular weight: 37.00 kDa

Protein length: 328 aa

Gene length: 984 bp

Function: potassium exporter

Product: potassium channel protein

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Potassium uptake/ export
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Regulation
• Lifestyles → Sporulation → Sporulation proteins → Newly identified sporulation proteins (based on transcription profiling)
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,218,854 → 3,219,840

Phenotypes of a mutant

• reduced biofilm formation (can be suppressed by inactivation of sinR) PubMed

The protein

Domains

• contains a RCK_N domain (according to UniProt)

Localization

• inner spore membrane PubMed

Additional information

• potassium exporter activity of YugO is activated by glutamate limitation via the C-terminal domain PubMed

Expression and Regulation

Operons

• Genes: mstX-yugO
  Description: PubMed

  Regulatory mechanism

  • SinR: repression, PubMed, in SinR regulon

  Regulation

  • repressed by SinR PubMed

  Additional information

  • potassium exporter activity of YugO is activated by glutamate limitation via the C-terminal domain PubMed

Biological materials

Mutant

• MGNA-A624 (yugO::erm), available at the NBRP B. subtilis, Japan
• TMB92 (yugO::tet), available in Erhard Bremer's and Jörg Stülke's labs
• GP2098 (ΔyugO::ermC), available in Jörg Stülke's lab PubMed
• BKE31322 (ΔyugO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGAAATAAATATCCGATTTG, downstream forward: _UP4_GAAACAGATCAATTCCTTGC
• BKK31322 (ΔyugO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGAAATAAATATCCGATTTG, downstream forward: _UP4_GAAACAGATCAATTCCTTGC

FLAG-tag construct

• GP2442 yugO-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab

References

Reviews

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Original publications

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